From the Flight Deck Video Series: Line Up and Wait
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“LINE UP AND WAIT” is used to instruct a pilot to taxi onto the departure runway and line up and wait, but NOT depart. While it may seem straightforward on the surface, there are numerous examples in which the instructions were not followed creating a dangerous risk of collision. A variety of human factors can explain why a pilot may mistakenly initiate a takeoff in this situation. An awareness and understanding of these factors along with strategies to mitigate those risks, will help you avoid a costly error. Learn more in this From the Flight Deck video from the FAA.
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